2018-06-29 17:26:112025-05-28 16:22:34
description
transcription elongation factor, resolves stalled [SW|RNA polymerase] at promoter or promoter-proximal regions,
transcription elongation factor, resolves stalled [SW|RNA polymerase] at promoter or promoter-proximal regions
locus
BSU27320
BSU_27320
geneLength
471
474
outlinks
bsu
BSU27320
BSU_27320
Gene
Coordinates
2,791,494 → 2,791,967
2,791,494 2,791,967
Gene
Phenotypes of a mutant
[SW|RNA polymerase] accumulates in promoter-proximal regions of many genes [Pubmed|21515770]
[SW|RNA polymerase] accumulates in promoter-proximal regions of many genes [Pubmed|21515770]
non-transformable [pubmed|28189581]
The protein
Protein family
greA/greB family (according to Swiss-Prot)
GreA/GreB family (single member, according to UniProt)
Biological materials
Mutant
BP351 ([[gene|greA]]::''cat'') available in [SW|Fabian Commichau]'s lab
GP2612 ([[gene|greA]]::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE27320 (Δ''[[gene|greA]]''::''ermC'') (available in the BGSC and in [SW|Jörg Stülke]'s lab)
BKE27320 (Δ[[gene|greA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCCTTCACTCCTTCT, downstream forward: _UP4_TAATATCTGTTTAGCAGCGA
BKK27320 (Δ[[gene|greA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCCTTCACTCCTTCT, downstream forward: _UP4_TAATATCTGTTTAGCAGCGA
BP351 ([[gene|greA]]::''cat'') available in [SW|Fabian Commichau]'s lab
GP2612 ([[gene|greA]]::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE27320 (''[[gene|greA]]''::''ermC'') (available in the BGSC and in [SW|Jörg Stülke]'s lab)
BKE27320 ([[gene|greA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCCTTCACTCCTTCT, downstream forward: _UP4_TAATATCTGTTTAGCAGCGA
BKK27320 ([[gene|greA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCCTTCACTCCTTCT, downstream forward: _UP4_TAATATCTGTTTAGCAGCGA
Biological materials
Expression vector
C-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP382]: pGP2547, available in [SW|Jörg Stülke]'s lab [pubmed|28189581]
References
Original Publications
The protein
Catalyzed reaction/ biological activity
resolves stalled [SW|RNA polymerase] at promoter or promoter-proximal regions
required for repair of DNA lesions during growth [pubmed|32041798]
promotes error-prone DNA repair during stress to promote genetic diversity [pubmed|32041798]
Biological materials
Expression vectors
C-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP382]: pGP2547, available in [SW|Jörg Stülke]'s lab [pubmed|28189581]